<b>Carragennan from <em>Hypnea musciformis</em> epiphyte obtained from <em>Solieria filiformis</em> experimental culture at Flecheiras, Ceará State, Brazil</b> - doi: 10.4025/actascitechnol.v33i2.9096

Abstract

Solieria filiformis (Rhodophyceae) culture is being developed at Flecheiras Beach, state of Ceará, Brazil, for its future sustainable use in biotechnology. However, epiphyte growth on the species jeopardizes suitable development. The efficiency of two methods (M I and M II) in the isolation of kappa-carrageenan (κ-CAR) from red marine alga Hypnea musciformis has been evaluated. κ-CAR was obtained by proteolytic digestion (M I) or by hot water (80°C) (M II) and extracts were analyzed with regard to their chemical composition and then by ion-exchange chromatography on DEAE-cellulose column with NaCl gradient. Anticoagulant activity was evaluated by the activated partial thromboplastine time (APTT) test with common human plasma and standard heparin (HEP) (193 IU mg^-1). Yield difference reached 4.28 ± 0.26%% (p < 0.05), and the lowest sulfate content was obtained with M I (17.00%) when compared to that with M II (26.30%). Further, different chromatographic profiles were reported between the two methods. Whereas M I was efficacious in obtaining κ-CAR anticoagulants (2.67 IU mg^-1), alternative M II was efficient for the food industry. Results suggest that κ-CAR extraction method from H. musciformis depends on the industry´s usage criterion.