Exploratory analysis of bioactive compounds and antioxidant potential of grape (Vitis vinifera) pomace

Autores

  • Leomara Floriano Ribeiro Universidade Federal do Paraná http://orcid.org/0000-0001-6610-0061
  • Rosemary Hoffmann Ribani Universidade Federal do Paraná
  • Ana Paula Stafussa Universidade Federal do Paraná
  • Cleyton Nascimento Makara Universidade de São Paulo
  • Ivanise Guilherme Branco Universidade Estadual Paulista
  • Giselle Maria Maciel Universidade Tecnológica Federal do Paraná
  • Charles Windson Isidoro Haminiuk Universidade Tecnológica Federal do Paraná

DOI:

https://doi.org/10.4025/actascitechnol.v44i1.56934

Palavras-chave:

Phenolic compounds; antimicrobial activity; winemaking; multivariate analyses; spectroscopy analyses.

Resumo

The aim of this study was to explore the profile of bioactive compounds of Merlot grape pomace by different methodologies including phenolic composition by spectrophotometry, chromatography and spectroscopy and its antibacterial activity. Additionally, the correlation between phenolic compounds and antioxidant activity by principal component analyses (PCA) was evaluated. Merlot grape pomace showed different classes of bioactive compounds such as phenolic acids, flavonoids and stilbene with high antioxidant activity according to the DPPH (2.58 ± 0.07 mg mL‑1), autoxidation of system β-carotene/linoleic acid (70.60 ± 0.91%), ABTS (1.22 Â± 0.01 mg mL-1) and FRAP (23.83 ± 0.64 µM FeSO4 g-1) assays. The extracts showed minimum inhibitory concentration for gram-positive and gram-negative bacteria with high values for Bacillus cereus (MIC = 12.5 mg mL-1) and Staphylococcus aureus (MIC = 25 mg mL-1), respectively. In addition, fingerprint regions of the phenolic compounds were found by mid-infrared, supporting the results of the chromatographic analyses. Through the PCA, the antioxidant potential assays had high correlation with phenolic composition. Therefore, considering the significant concentrations of the different bioactive compounds found in Merlot grape pomace, it can be inferred that this cheap by-product can be reused by the food industry in the development of new products

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Publicado

2022-01-12

Como Citar

Ribeiro, L. F., Ribani, R. H. ., Stafussa, A. P. ., Makara, C. N. ., Branco, I. G. ., Maciel, G. M. ., & Haminiuk, C. W. I. . (2022). Exploratory analysis of bioactive compounds and antioxidant potential of grape (Vitis vinifera) pomace. Acta Scientiarum. Technology, 44(1), e56934. https://doi.org/10.4025/actascitechnol.v44i1.56934

Edição

Seção

Ciência, Tecnologia de Alimentos e Engenharia de Alimentos

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