Evaluation of soybean cultivars on the embryogenic and organogenic potential - doi: 10.4025/actasciagron.v33i1.12344

Authors

  • Leandra Regina Texeira UEM Author
  • Alessandro De Lucca e Braccini UEM Author
  • Bruno Grimaldo Martinho Churata UFPR Author
  • Elisa Serra Negra Vieira Cooperativa Central de Pesquisa Agrícola Author
  • Polyana Kelly Martins Cooperativa Central de Pesquisa Agrícola Author
  • Ivan Schuster Cooperativa Central de Pesquisa Agrícola Author

DOI:

https://doi.org/10.4025/actasciagron.v33i1.12344

Keywords:

Glycine max, somatic embryogenesis, organogenesis

Abstract

The first step in plant transformation using somatic embryogenesis or organogenesis for plant regeneration in vitro has been the identification of genotypes with the best totipotency. Thus, the objective of this experiment was to evaluate the totipotency of ten cultivars and one soybean line from the Coodetec Gene Bank. The somatic embryos developed from immature cotyledons measuring from 4 to 6 mm in length, and the organogenesis was evaluated from mature soybean seeds. The cultivars CD 201, CD 216 and CD 220 were the most promising genotypes to produce somatic embryos, whereas the cultivar CD 217 had the highest quantity of differentiated explants, which represents the best regeneration efficiency for participating in organogenic systems. In the experiment with somatic embryogenesis, time to plant regeneration was ten months, but the plants were regenerated after only four months in the experiment with organogenesis.

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Published

2011-02-16

Issue

Vol. 33 No. 1 (2011)

Section

Genetics and Plant Breeding

License

DECLARATION OF ORIGINALITY AND COPYRIGHTS

I Declare that current article is original and has not been submitted for publication, in part or in whole, to any other national or international journal. 

The copyrights belong exclusively to the authors. Published content is licensed under Creative Commons Attribution 4.0 (CC BY 4.0) guidelines, which allows sharing (copy and distribution of the material in any medium or format) and adaptation (remix, transform, and build upon the material) for any purpose, even commercially, under the terms of attribution.

 

 

How to Cite

Evaluation of soybean cultivars on the embryogenic and organogenic potential - doi: 10.4025/actasciagron.v33i1.12344. (2011). Acta Scientiarum. Agronomy, 33(1), 67-74. https://doi.org/10.4025/actasciagron.v33i1.12344
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