<b>Kinetics of the simultaneous production of b- and g-cyclodextrins catalyzed by CGTase from alkalophilic <i>Bacillus</i> sp.</b> - doi: 10.4025/actascitechnol.v35i4.13944

Autores

  • Marcos De Souza Universidade Estadual de Maringá
  • Sérgio Henrique Bernardo de Faria Universidade Estadual de Maringá
  • Gisella Maria Zanin Universidade Estadual de Maringá
  • Flavio Faria Moraes Universidade Estadual de Maringá

DOI:

https://doi.org/10.4025/actascitechnol.v35i4.13944

Palavras-chave:

cyclic oligosaccharides, cyclization, enzyme kinetics, inclusion complexes, , intramolecular transglycosylation

Resumo

The cyclodextrins (CDs) are cyclic maltooligosaccharides obtained by cyclization of linear chains of starch, catalyzed by the enzyme cyclomaltodextringlucanotransferase (CGTase). The interest in CD production results from the formation of inclusion complexes, which allow many important applications, especially in food, pharmaceutical and cosmetic industries. The substances complexed generally have their properties modified by complexation. It is appreciated if increased solubility and higher thermal and chemical stabilities are obtained. In this work, a kinetic model was developed for the production of cyclodextrins in the presence of CGTase from alkalophilic Bacillus sp., taking into account the reversibility of the cyclization reaction, the simultaneous production of b and g-CD and also the inhibitory influence of the substrate and products (CDs), on the enzymatic activity of the CGTase. The substrate formed from a solution of maltodextrins was treated as a single substrate. The model was compared with experimental results of 24h of reaction and this comparison demonstrated that there was a very good representation of the data throughout the test period. The model also allowed explaining the observation of different experimental values for each Michaelis-Menten constant and substrate inhibition constant for each CD, although the CDs are produced from the same substrate.

 

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Biografia do Autor

Marcos De Souza, Universidade Estadual de Maringá

Atualmente é professor da Universidade Estadual de Maringá. Tem experiência na área de Engenharia Quimica, com ênfase em Cinética Enzimática e Modelagem Matemática, atuando principalmente nos seguintes temas: ciclodextrina, glicosiltransferase, cinética, CGTase , modelagem, CFD, Reatores Microestruturados. "http://lattes.cnpq.br/7769970379087115"

Sérgio Henrique Bernardo de Faria, Universidade Estadual de Maringá

Departamento de Engenharia Quí­mica

Gisella Maria Zanin, Universidade Estadual de Maringá

Departamento de Engenharia Quí­mica

Flavio Faria Moraes, Universidade Estadual de Maringá

Departamento de Engenharia Quí­mica

Publicado

2013-05-22

Como Citar

De Souza, M., Faria, S. H. B. de, Zanin, G. M., & Moraes, F. F. (2013). <b>Kinetics of the simultaneous production of b- and g-cyclodextrins catalyzed by CGTase from alkalophilic <i>Bacillus</i> sp.</b> - doi: 10.4025/actascitechnol.v35i4.13944. Acta Scientiarum. Technology, 35(4), 687–693. https://doi.org/10.4025/actascitechnol.v35i4.13944

Edição

Seção

Engenharia Quí­mica

 

0.8
2019CiteScore
 
 
36th percentile
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0.8
2019CiteScore
 
 
36th percentile
Powered by  Scopus